Fig. 5

SNCG is an indicator of adverse prognosis and positively correlates with activated β1 integrin, p-FAK (Y³⁹⁷) in CRC tissues. a-b, Kaplan-Meier estimation of disease-free survival (DFS) for stage I-II (a) and III-IV (b) colorectal adenocarcinoma patients according to SNCG levels. c, Correlations of SNCG levels in CRC tissues with post-operative recurrence and status. d, Representative blots from three independent experiments were presented. Protein levels of SNCG, activated β1 integrin, and p-FAK (Y³⁹⁷) in clinical colon cancer tissue samples were evaluated by Western blot analysis. In order to increase the reproducibility, HCT116 cell lysates were used in each blot as the internal control (c) to minimize the effect of band intensity variation. GAPDH was used as the loading control. e-g, Correlation between the relative protein levels of activated β1 integrin levels and p-FAK (Y³⁹⁷) (e), SNCG and active β1 integrin (f), and SNCG and p-FAK (Y³⁹⁷) (g) were plotted as a scatter plots