Fig. 2

Effects of Lycorine on proliferation, migration and colony formation of GBM cells in vitro. a Chemical structure of Lycorine. b U251 cells were treated with indicated concentrations of Lycorine (0, 1, 5, 10, 25, 50 μM) for 48 h. Cell viability was assessed by SRB assay (n = 3) and photos of the cell morphology were taken by microscope at the light field. c Statistical result of Fig. 2b. d U251 cells were seeded on the upper chamber of Transwell. After 5 to 7 h incubation with Lycorine (from 0 μM to 10 μM), migrated cells were fixed and stained. The number of migrated cells were calculated. e Statistical result of Fig. 2d. f Cells were seeded in 6-well plates for 7 days after the treatment of Lycorine in according concentrations and fixed with 4% paraformaldehyde, and stained with 0.2% crystal violet. The statistical results of colony numbers and diameters were presented in g and h. All data are represented as mean ± S.D. from triplicate wells. *, p < 0.05, **, p < 0.01, ***, p < 0.001, as compared to control