Fig. 5

TrkAIII preconditions NB cells to better survive and resolve acute ER stress. Representative RT-PCR agarose gels comparing DTT induced Xbp-1 splicing, CHOP and Grp78/Bip expression in control, fully spliced TrkA and TrkAIII SH-SY5Y transfectants, demonstrating that TrkAIII transfectants exhibit complete attenuation of DTT-induced Xbp-1 splicing and CHOP but not Grp78/Bip expression within 12 h, not observed in either control or TrkA transfectants plus the prevention of this effect in TrkAIII transfectants pre-incubated for 1 h with 100 nM CEP-701 prior to DTT treatment