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Table 1 TaqMan assays (primer and probe sets) employed in dPCR, RT-ddPCR and RT-experiments

From: Liquid biopsy in mice bearing colorectal carcinoma xenografts: gateways regulating the levels of circulating tumor DNA (ctDNA) and miRNA (ctmiRNA)

Assay (primers and probe) Assay codea Employed technology
KRAS p. G12D KRAS_521, ID AH6R5PIb dPCR
KRAS p. G13D KRAS_ 532, ID AHD2BW0b dPCR
PIK3CA p. H1047R PIK3CA_775, ID AHPAVCDb dPCR
FBXW7 p. R505C custom assay, ID ANZTGJUb dPCR
hsa-miR-141-3p 000463c RT-ddPCR, RT-qPCR
hsa-miR-221-3p 000524c RT-ddPCR, RT-qPCR
hsa-miR-222-3p 002276c RT-ddPCR, RT-qPCR
cel-miR-39-3p 000200c RT-ddPCR, RT-qPCR
  1. aAll assays are provided from Thermo Fisher Scientific. bThis assay was used for dPCR on genomic DNA and ctDNA. cThis assay was used for reverse transcription and amplification of microRNAs in ddPCR and quantitative PCR