Journal of Experimental & Clinical Cancer Research

Table 1 TaqMan assays (primer and probe sets) employed in dPCR, RT-ddPCR and RT-experiments

From: Liquid biopsy in mice bearing colorectal carcinoma xenografts: gateways regulating the levels of circulating tumor DNA (ctDNA) and miRNA (ctmiRNA)

Assay (primers and probe)	Assay code^a	Employed technology
KRAS p. G12D	KRAS_521, ID AH6R5PI^b	dPCR
KRAS p. G13D	KRAS_ 532, ID AHD2BW0^b	dPCR
PIK3CA p. H1047R	PIK3CA_775, ID AHPAVCD^b	dPCR
FBXW7 p. R505C	custom assay, ID ANZTGJU^b	dPCR
hsa-miR-141-3p	000463^c	RT-ddPCR, RT-qPCR
hsa-miR-221-3p	000524^c	RT-ddPCR, RT-qPCR
hsa-miR-222-3p	002276^c	RT-ddPCR, RT-qPCR
cel-miR-39-3p	000200^c	RT-ddPCR, RT-qPCR

^aAll assays are provided from Thermo Fisher Scientific. ^bThis assay was used for dPCR on genomic DNA and ctDNA. ^cThis assay was used for reverse transcription and amplification of microRNAs in ddPCR and quantitative PCR

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ISSN: 1756-9966

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