Fig. 1
From: Metabolic reprogramming-based characterization of circulating tumor cells in prostate cancer
Screening and validation of metastasis-related metabolic genes in PCa cell lines. a Hierarchical clustering analysis of the metabolic gene profiles between 2B4 and 1E8 cells in the functional microarray assay. b The array identified genes that were up-regulated in 1E8 cells compared with 2B4 cells (change fold > 1.5 and P < 0.05). c–d Relative mRNA levels of the differentially expressed metabolic genes in c 2B4 and 1E8 cells, and d LNCAP, PC-3, and DU145 cells by qRT-PCR test, including HK2, PDP2, G6PD, PGK1, PHKA1, PYGL, PDK1, and PKM2 (*P < 0.05). e Protein levels of the metabolic genes in all five PCa cells by Western blot and quantitative analysis (*P < 0.05 between 1E8 and 2B4; #P < 0.05 among LNCAP, PC-3, and DU145)