Fig. 2

AZD1775 facilitates olaparib-induced apoptosis in GC cells. a-c Apoptosis analysis using Annexin V-PE/7-AAD double-staining and immunoblots with anti-caspase 3 and Bax antibodies were carried out in MKN45 and AGS cells treated with 0.3 μM AZD1775 in the presence or absence of 20 μM olaparib for 48 h. AZD, AZD1775; Ola, olaparib; Com, Combination. Data expressed as Mean ± SD and representative of three independent experiments. *, P < 0.05 by ANOVA analysis