Fig. 4

COPS3 loss suppresses EMT through inhibition of RSK (a) Western blotting analyzes the levels of RSK and its phosphorylated form, N-cadherin, E-cadherin, vimentin and MMP-9 in 143B and HOS cells stably transfected with shCOPS3 or shCtrl. b SAOS-2 cells were transfected with COPS3 adenovirus. The cell lysates were analyzed by immunoblotting with indicated antibodies. c Osteosarcoma cells were treated with 5 μM or 10 μM RSK inhibitor BI-D1870 for 24 h, cells lysates were subjected to immunoblotting using indicated antibodies. d and e 143B cells were exposed to 5 μM or 10 μM BI-D1870 for 24 h, migratory and invasive abilities were analyzed by transwell assay (d) and wound healing assay (e). Scale bars: 100 μm. Data representing mean ± S.D. were from three experiments with similar results, **P < 0.01 vs. control group