Fig. 5

COPS3 silencing suppresses osteosarcoma metastasis in vivo. a and b Each group of mice were injected with 143B-shCtrl or 143B-shCOPS3 cells through tail vein (mean ± SD, n = 6). After four weeks, tumor nodules of lung metastasis were presented with representative images and H&E staining of lungs. c COPS3 expression in xenograft was detected by Real-time RT-PCR. d Variations of indicated proteins in vivo were analyzed by Western blotting. **P < 0.01 vs. shCtrl group. Scale bars: 100 μm