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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Novel oncogene COPS3 interacts with Beclin1 and Raf-1 to regulate metastasis of osteosarcoma through autophagy

Fig. 6

COPS3 inhibition diminishes the autophagic flux. a 143B cells lysates were co-immunoprecipitation with anti-COPS3 and probed with anti-Beclin1(left), cytoplasm extracts and nuclear extracts of 143B or HOS cells were separated and collected for co-immunoprecipitation (right). b 143B or HOS cells were exposed to 1 mM H2O2 for 4 h, the cell lysates were co-immunoprecipitation with anti-COPS3 and probed with anti-LC3-I/II. c 143B cells were transfected with siCOPS3 for 48 h, followed by treated with 1 mM H2O2 for 4 h in the presence or absence of CQ (8 μM). Indicated proteins were detected by immunoblotting and the relative levels of LC3-II were shown below. d Punctate LC3 of Mock and COPS3 silencing 143B cells were presented and quantified. Scale bars: 10 μm. e 143B cells were treated with COPS3 or Beclin1 siRNA. Indicated proteins expression were evaluated by immunoblotting. f 143B cells were exposed to 3-MA (1 and 2 mM) or Beclin1 siRNA. The migratory and invasive abilities were analyzed by transwell assays. Results above are expressed as the mean ± S.D. of three independent experiments. Scale bars: 100 μm **P < 0.01 vs. Mock or control group

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