Fig. 4
LncGPR107 drove the expression of GPR107. a GPR107 expression levels in lncGPR107 ASO treated cells were detected through realtime PCR. b, c GPR107 expression levels in lncGPR107 depleted cells (b) or lncGPR107 overexpressing cells (c) were examined by Western blot. β-actin was a loading control. d GPR107 deleted cells were established through CRISPR/Cas9 for sphere formation. Western blot was performed for deletion confirmation (left panels). Typical sphere images and sphere initiating cell ratios were shown in middle and right panels, respectively. e 10, 1 × 102, 1 × 103, 1 × 104 and 1 × 105 GPR107 deficient cells were used for 3 months’ tumor initiation. The ratios of tumo-free mice were shown in left panels and TICs ratios were shown in right panels. n = 6 for each group. f, g LncGPR107 was silenced (f) or overexpressed (g) in GPR107 deleted cells, and sphere formation assay was performed. h, i Transwell assay of the indicated cells revealed that lncGPR107 had impaired role upon GPR107 knockout. Experiments were repeated three times