Fig. 6
LncGPR107-SRCAP-GPR107 can be targeted for liver TIC elimination. a 1 × 106 primary cells were injected into BALB/c nude mice. When the volume of tumors were reach about 250 mm3, lncGPR107 ASO (antisense oligo) and control ASO were injected into tumors every two days. Tumor weight was measured one month later. n = 6 for each group. b 1 × 106 lncGPR107 overexpressing cells were used for tumor propagation. n = 6 for each group. c 1 × 106 indicated cells were used for tumor propagation, and the tumors were weighted one month later. n = 6 for each group. d The indicated tumors were obtained and GPR107 expression was examined through immunohistochemistry. Scale bars, 100 μm. e, f The indicated tumors were obtained and separated into individual cells for FACS. Ki67+ cells (e) and CD133+ liver TICs (f) were examined. n = 6 for each group, and the ratios of Ki67+ and CD133+ cells were shown as means±s.d. Experiments were repeated three times