Fig. 1

PTEN phosphatase inactivation is related to GC development. a IHC staining of p-PTEN in GC and ANTT tissues. b Kaplan–Meier analysis of p-PTEN expression and survival time in GC patients (n = 90; p < 0.05, log-rank test). c, f MTT assay, d, g BrdU assay, e, h colony-formation assay, and i, j high content analysis were performed to detect the proliferation of BGC823 and SGC7901 stable cells expressing wild-type PTEN, dominant-negative mutant type PTEN C124S (Mut 1) or G129E (Mut 2) or an empty vector. k, m Transwell and l, n high content analysis were conducted to detect the migration ability of the stable cells. The means±SEMs of a representative experiment (n = 3) performed in triplicate are shown. *, p < 0.05; **, p < 0.01