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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: PTEN lipid phosphatase inactivation links the hippo and PI3K/Akt pathways to induce gastric tumorigenesis

Fig. 4

The Hippo pathway is involved in the malignant biological behaviors of GC that are induced by PTEN lipid phosphatase inactivation. a, d Immunoblot of upstream molecules and (p)-YAP/TAZ in the Hippo pathway in stable cells expressing wild-type PTEN, dominant-negative mutant type PTEN C124S (Mut 1) or G129E (Mut 2) or empty vector. b, e Immunoblot of YAP/TAZ expression levels in nuclear and cytoplasmic proteins of the stable cells. c, f Immunofluorescence analysis of YAP (red) and DAPI (blue) in stable cells. g, h Coimmunoprecipitation analysis of MOB1-LATS1/2 by incubating with an anti-MOB1 antibody in stable cells. i, j Cell viability of the cells after treatment with 0, 0.5, 1, 5, and 10 μM VP for 72 h. k, l Relative distance of cell motility after treatment with 0, 1, and 10 μM VP for 72 h. m, n Quantification of tumor volumn *, p < 0.05; **, p < 0.01

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