Fig. 5
From: ER-α36 mediates cisplatin resistance in breast cancer cells through EGFR/HER-2/ERK signaling pathway
Increased activation of ER-α36-mediated nongenomic estrogen signaling is responsible for cisplatin resistance. a, b MCF-7 and MCF-7/DDP cells (a) or MCF-7/V and MCF-7/ER-α36 cells (b) were harvested and the protein levels of ER-α36, EGFR, HER-2, total ERK (ERK) and phosphorylated ERK (P-ERK) were detected using western blot. c, d MCF-7/DDP (c) and MCF-7/ER-α36 (d) cells were transfected with siER-α36 and negative control siRNA (siNC) for 48 h. Then the cells were collected and the levels of ER-α36, EGFR, HER-2, P-ERK, ERK were analyzed by western blot