Fig. 3

The influence of the chemoimmunotherapy on infiltration and activity stage of myeloid subpopulations infiltrating MC38 tumors. a The scheme of the multiparameter flow cytometric analysis showing the way of distinguishing myeloid cell subpopulations; b The percentage of leukocytes; c Representative histograms presenting changes in MHC II and CD80 expression in selected cells after treatment with shIL10–3 LVs; d The percentage of myeloid CD11b⁺ cells; e M1/M2 ratio showing changes in polarization of tumor infiltrating macrophages occurring during therapy; f, g subpopulations of myeloid cells and their activation stage after therapy. To calculate the mean ± SD at least 5 mice per group were analyzed. The differences between the groups were estimated using nonparametric Kruskal-Wallis test followed by Dunn’s multi comparison test (* p < 0.05, ** p < 0.01, *** p < 0.001)