Fig. 2

SGK3 enhances the expansion of liver CSCs. a, b Relative mRNA expression of liver CSC-related markers CD133, CD90, Nanog, Oct4, Bmi-1 and Sox2 in Huh7 and MHCC-97H cells stably overexpressing SGK3 or NC. β-actin was used as an loading control. c Expression of CD133, Nanog and SGK3 in Huh7 and MHCC-97H cells stably overexpressing SGK3 or NC detected by western blot. β-actin was used as a loading control. d Spheroid formation assay of MHCC97H-SGK3 or MHCC97H-NC cells (top). Scale bars, 200 μm. The statistical results of the tumour spherosis (> 50 μm) were calculated based on 3 independent experiments (bottom). All experiments were performed in triplicate, and the results are shown as mean ± standard deviation. *P < 0.05