Fig. 2

UFC1 knockdown inhibits gastric cancer cell proliferation, migration and invasion. a UFC1 expression was silenced in gastric cancer cells by using shRNA. The efficiency of gene knockdown was validated. b The growth of sh-UFC1 transfected GC cells was determined by using cell counting assay. c Cell colony formation assays for the proliferating ability of sh-UFC1 transfected GC cells. d The cell cycle distribution in sh-UFC1 transfected GC cells was determined by using flow cytometry. e Flow cytometric analyses of cell apoptosis in sh-UFC1 transfected GC cells. f The effects of UFC1 knockdown on the migration of GC cells were determined by using transwell migration assay. g Matrigel invasion assay was performed to determine the effects of UFC1 knockdown on GC cell invasion. h The effects of UFC1 knockdown on the expression of growth and metastasis-related genes in GC cells. i The effects of UFC1 knockdown on the expression of growth and metastasis-related proteins in GC cells