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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Long non-coding RNA UFC1 promotes gastric cancer progression by regulating miR-498/Lin28b

Fig. 3

UFC1 interacts with miR-498 in gastric cancer cells. a The potential binding site of miR-498 in UFC1 was predicted by using bioinformatic analyses. b QRT-PCR analyses of UFC1 expression in GC cells transfected with miR-498. c GC cells were co-transfected with UFC1 3’-UTR reporter plasmid wild type or mutant with miR-498 or miR-498 antago. The luciferase activity was determined by using dual-luciferase reporter assay. d GC cells transfected with vector EGFP or miR-498 were treated with actinomycin D for different times. The relative expression levels of UFC1 were determined by using qRT-PCR. e RIP assay for the binding of UFC1 with Ago2 protein. f The expression of miR-498 in gastric cancer tissues was examined by using qRT-PCR. The association between miR-498 and UFC1 expression level was determined by using Pearson correlation analysis. g The prognostic values of miR-498 expression level in gastric cancer

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