Fig. 5

Lin28b is a downstream target ofmiR-498. a The potential binding site in Lin28b for miR-498 was predicted by using bioinformatic analyses. b QRT-PCR analyses of Lin28b expression in GC cells transfected with miR-498. c The expression of Lin28b in gastric cancer tissues was examined by using qRT-PCR. The association between Lin28b and miR-498 expression levels was determined by using Pearson correlation analysis. d GC cells were co-transfected with Lin28b 3’-UTR reporter plasmid wild type or mutant with miR-498 plasmid or miR-498 antago. The luciferase activity was determined by using dual-luciferase reporter assay. e GC cells were transfected with miR-498 in the presence or absence of Lin28b. The proliferating ability of GC cells was determined by using cell colony formation assay. f Cell cycle distribution was determined by using flow cytometry. g Cell apoptosis was determined by using flow cytometry. h The migration ability of GC cells were determined by using transwell migration assay. i The invasion ability of GC cells were determined by using matrigel invasion assay