Fig. 4

E2 regulates IL6 expression through ERβ and affects malignancy of lung cancer. (a) Autocrine IL6 was analyzed by ELISA assay after overexpression or knockdown of ERβ. (b) Upregulation of IL6 induced by E2 (10 nM) treatment 48 h was determined by immunofluorescence in A549. (c) Colony formation assay measuring the proliferative activity in A549 cells after overexpression or knockdown of ERβ. (d) Wound-healing assays were performed to assess NSCLC cell migration in response to changes in ERβ expression. (e-f) Transwell assay and DQ-collagen invasion assay were used to quantify the effect of ERβ regulation on cell migration and invasion capacity. (g) Expression of IL6 by western blotting after modulation of ERβ expression