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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: 17β-estradiol upregulates IL6 expression through the ERβ pathway to promote lung adenocarcinoma progression

Fig. 5

Upregulation of IL6 stimulated by E2 induces tumorigenesis in a urethane-induced adenocarcinoma model. (a) The process of creating a urethane-induced adenocarcinoma model illustrated in a simplified sequence flow diagram. (b-d) Two weeks after urethane was injected, mice were randomly divided into 3 groups (N = 6/group): E2 (0.09 mg/kg), E2+ Ful (1.46 mg/kg) and blank control. Lungs were removed after 12 weeks of subcutaneous drug treatment. The gross appearance of lung tumor nodes in different groups is indicated by black circles. The tumor numbers of different groups and tumor growth curves were obtained. (e-f) IHC staining of ERβ and IL6 shows a significant positive linear correlation between ERβ and IL6 expression. (g) Protein expression of ERβ, IL6, p-p38MAPK, p-AKT and p-Stat3 in murine lung tumors was analyzed using western blot. All data are expressed as the mean ± SD. Student’s t-test was used for statistical analysis

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