Fig. 1

AKBA inhibits cell proliferation and increases cytotoxicity in a dose- and time-dependent manner in U251 and U87-MG cells. a Morphology of cells treated with AKBA at 10, 20, and 30 μM. Scale bar = 100 μm. b CCK8 assay shows that AKBA inhibits proliferation of U251 and U87-MG cells. c LDH assay shows that AKBA is cytotoxic to U251 and U87-MG cells. d EdU-DNA synthesis assay sho ws that AKBA inhibits DNA synthesis in U251 cells. Scale bar = 50 μm. e EdU-DNA synthesis assay shows that AKBA inhibits DNA synthesis in U87-MG cells. Scale bar = 50 μm. The experiments were performed in triplicate, and the data are presented as mean ± SD, *P < 0.05 vs. control group