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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Ajuba inhibits hepatocellular carcinoma cell growth via targeting of β-catenin and YAP signaling and is regulated by E3 ligase Hakai through neddylation

Fig. 3

Hakai interacts with Ajuba through its HYB domain. a, b 293 T cells transfected with Myc-tagged Ajuba alone, GFP-tagged Hakai alone or both in combination. Cell lysates were subjected to immunoprecipitation (IP) with anti-Myc-tag (a) or anti-GFP-tag antibody (b) and immnoblotted (IB) with the indicated antibodies. c Detection of interaction between endogenous Hakai and Ajuba by IB at in BEL7402 cells (d, e). Schematic showing Hakai deletion mutants and their relative abilities to interact with Ajuba in transfected 293 T cells. f Co-IP of 293 T cells showing the domain of Ajuba binding to Hakai. IB, immnoblot. IP, immunoprecipitation. WCL, Whole-cell lysates

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