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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Ajuba inhibits hepatocellular carcinoma cell growth via targeting of β-catenin and YAP signaling and is regulated by E3 ligase Hakai through neddylation

Fig. 4

Hakai regulates Ajuba protein stability in HCC cells. a, b Immublotanalysis of Ajuba protein levels in Hakai-depleted or control HCC cells (A: BEL7402; B: HepG2) in the presence of cycloheximide (CHX, 80 μg/ml) for the indicated times, right panels showing the quantification of Ajuba relative protein levels. GAPDH was used as a loading control. c, d HCC cells were infected with controls or Hakai adenovirus and treated with CHX for the indicated times. Ajuba protein levels were determined by immunoblotting and quantified. (c: BEL7402; d: HepG2). GAPDH was used as a loading control. Data are presented as Mean ± SEM from three independent experiments (**p < 0.01, ***p < 0.001)

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