Fig. 4

MAP4K4 is a novel target of miR-98-5p. a MAP4K4 was predicted as a direct target of miR-98-5p. b The expression of MAP4K4 protein in PDAC cells after miR-98-5p alteration by western blot. c The expression of MAP4K4 mRNA in PDAC cells after miR-98-5p alteration by qRT-PCR. d Luciferase reporter assay was employed to confirm that miR-98-5p directly bound to the 3’UTR region of MAP4K4. e Luciferase activity was analyzed in CFPAC-1 cells co-transfected with miR-98-5p-mimics or control with MAP4K4-WT or MAP4K4-MUT. f Luciferase activity was detected in MIAPACA-2 cells co-transfected with miR-98-5p-inhibitor or negative control with MAP4K4-WT or MAP4K4-MUT. g, h Negative correlation between the expression levels of miR-98-5p and MAP4K4 in PDAC tissues. *P < 0.05, **P < 0.01. The data expressed as the mean ± SD. All experiments were repeated at least three times