Fig. 1

STK25 suppresses CRC cell proliferation. a Expression of STK25 mRNA in 4 CRC cell lines (SW480, RKO, LoVo, HCT116) was measured by qRT-PCR. b Expression of STK25 at the protein level in CRC cells was examined by western blot. Ratios of STK25:β-actin shown under the representative blots were normalized to STK25 in SW480 cells. Efficiency of STK25 overexpression and knockdown in LoVo and RKO cell lines were measured by qRT-PCR (c) and western blot (d). e Overexpression of STK25 in CRC cell lines significantly inhibits cell growth. f Depletion of STK25 significantly promotes cell proliferation. g STK25 overexpression attenuates colony formation in CRC cell lines. h Knockdown of STK25 increases colony formation in CRC cell lines. *, P < 0.05