Fig. 4

STK25 interacts with GOLPH3 and regulates its expression. a, b Exogenous STK25 interacts with GOLPH3. Cells were transfected with the indicated plasmids. Co-IP was performed using FLAG antibody to pull down FLAG-STK25 (a) or anti-Myc against Myc-GOLPH3 (b). Then, STK25 and GOLPH3 were detected with the indicated antibodies. c, d His-STK25 interacts directly with GST-GOLPH3 but not GST by in vitro GST pull-down and His-tag pull-down assays, respectively. e STK25 overexpression decreases GLOPH3 mRNA and protein levels in CRC cells. f STK25 knockdown increases GOLPH3 mRNA and protein levels in CRC cells