Fig. 7
STK25 knockdown promotes tumor growth and glycolytic gene expression in a xenograft mouse model. a The stable knockdown of STK25 by a shRNA construct in LoVo cells was confirmed by qRT-PCR and western blot. b Representative images of tumor-bearing mice implanted with LoVo-shSTK25 or LoVo-shControl cells (upper panel). Tumor masses were harvested from the corresponding xenografts on Day 28 (lower panel). Tumor volumes (c) and tumor weights (d) were measured on the indicated days. e Lysates from tumor masses harvested from the indicated mice were analyzed by western blot for the expression of glycolytic genes. f MicroPET/CT imaging of mice. Representative PET/CT photographs of animals (left panel). Knockdown of STK25 promotes 18F-FDG uptake in mice bearing LoVo xenograft (right panel). Data are expressed as mean ± SD. *, P < 0.05. **, P < 0.01