Fig. 1

Identification of ATF2 as a novel SPOP Interactor. a and b Western blot of whole cell lysates(WCL) and co-IP samples of anti-FLAG antibody obtained from 293 T cells transfected with indicated plasmids. c Western blot of WCL and co-IP samples of anti-FLAG antibody obtained from C4–2 cells infected with lentivirus expressing FLAG-SPOP or control. The cells were treated with 20 μM MG132 for 8 h before harvesting. d Western blot of co-IP samples of IgG or anti-ATF2 antibodies obtained from cell lysates of C4–2 cells. The cells were treated with 20 μM MG132 for 8 h before harvesting. e Schematic representation of SPOP deletion mutants. Binding capacity of SPOP to ATF2 is indicated with the symbol. f Western blot of WCL and co-IP samples of anti-FLAG antibody obtained from 293 T cells transfected with indicated plasmids