Fig. 4

Prostate cancer-associated mutants of SPOP are defective in promoting ATF2 degradation and ubiquitination. a Distribution of the most common mutations in the SPOP gene found in prostate cancer. b Western blot of WCL of 293 T cells transfected with indicated plasmids. c Western blot of WCL and co-IP samples of anti-FLAG antibody obtained from 293 T cells transfected with indicated plasmids. d Western blots of the products of in vivo ubiquitination assays performed using cell lysate from 293 T cells transfected with the indicated plasmids and treated with 20 μM MG132 for 8 h. e Western blot of WCL and co-immunoprecipitation of anti-FLAG antibody in lysates from 293 T cells transfected with the indicated plasmids. f Western blot of the in vivo ubiquitination assay in 293 T cells transfected with the indicated plasmids and treated with 20 μM MG132 for 8 h