Fig. 2

Inhibition of NSCLC cell proliferation and apoptosis by silencing of CPNE1. a CPNE1 mRNA and protein levels in CPNE1-silenced NSCLC cells. b CCK-8 assay of cell viability in NSCLC cell lines; cell viability was determined at 24, 48 and 72 h. c Representative images of the results of clonogenic analysis of cell proliferation in NSCLC cells. Bar charts showing clonogenic growth of cells. d Flow cytometry assay of A549 and H1299 cells (silenced for CPNE1 and compared with NC). Cells were harvested at 72 h after transfection and stained with Annexin V/FITC and propidium iodide (PI). e-f Flow cytometric analysis of NSCLC cell lines (CPNE1-silenced cells vs. NC cells). Cells were harvested at 72 h after transfection and stained with PI. The percentage of cells in each cell cycle phase is shown in the inset of each panel, in which the values represent the mean ± SD of three measurements. *P < 0.05; **P < 0.01; ***P < 0.001