Fig. 3

CPNE1 silencing-induced inhibition of the migratory and invasive abilities of NSCLC cells and their associated pathways. a The wound healing assay showed that the speed with which cells migrated towards the scratch was lower in CPNE1-silenced cells than in control cells. b CPNE1 silencing inhibited invasion and migration of NSCLC cells. CPNE1-silenced NSCLC cells were allowed to migrate through an 8-μM pore Transwell. The cells that migrated were stained and counted in at least three microscopic fields (magnification, × 100). Then, the cells were treated as above and allowed to invade through the Matrigel-coated membrane in Transwells. Invasive cells were stained and counted under a light microscope. c Co-immunoprecipitation of Copine-1 and EGFR. Copine-1 was immunoprecipitated from lysates of control and CPNE1-overexpressing H1299 cells using a specific monoclonal antibody. d p-EGFR and downstream signaling molecules were detected, and the data showed that the p-EGFR, p-Src, p-FAK, p-AKT and p-ERK levels were significantly decreased in the CPNE1-silenced cells compared with the control cells. e In the stable cell lines with CPNE1 knockdown, the EGF-induced increase in the level of p-EGFR and the other downstream signaling molecules levels was inhibited. *P < 0.05; **P < 0.01; ***P < 0.001