Fig. 5

Upregulation of miR-133a-3p attenuates anoikis resistance in PCa cells. a Annexin V-FITC/PI staining of the indicated cells. Error bars represent the mean ± S.D. of three independent experiments. *P < 0.05. b The JC-1 staining in the indicated cells. Error bars represent the mean ± S.D. of three independent experiments. *P < 0.05. c and d Analysis of the activities of caspase-3 (c) and caspase-9 (d) were detected by the cleaved forms of these two proteins. Error bars represent the mean ± S.D. of three independent experiments. *P < 0.05. e Western blotting analysis of Bcl-2, Survivin, Mcl-1 and Bcl-xL in the indicated cells. α-Tubulin served as the loading control