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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Downregulation of miR-133a-3p promotes prostate cancer bone metastasis via activating PI3K/AKT signaling

Fig. 6

miR-133a-3p targets EGFR, FGFR1, IGF1R and MET. a Predicted target of miR-133a-3p in TargetScan, miRwalk and miRanda. b Real-time PCR analysis of EGFR, ERBB4, FGFR1, IGF1R, IFG2R, IN2R, MET and NGFR expression in the indicated PCa cells. Transcript levels were normalized by GAPDH expression. Error bars represent the mean ± s.d. of three independent experiments. *P < 0.05. c Western blotting of EGFR, FGFR1, IGF1R and MET expression in the indicated cells. α-Tubulin served as the loading control. d-f Luciferase assay of cells transfected with pmirGLO-3′UTR reporter in the indicated PCa cells, respectively. *P < 0.05. g-i MiRNP IP assay showing the association between miR-133a-3p and EGFR, FGFR1, IGF1R and MET transcripts in PCa cells. Pulldown of IgG antibody served as the negative control. *P < 0.05

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