Fig. 7

miR-133a-3p negatively regulates EGFR, FGFR1, IGF1R and MET expression, and PI3K/AKT signaling activity. a Western blotting of p-AKT expression in the indicated cells at S473 and T308. α-Tubulin served as the loading control. b Luciferase reporter analysis of AKT signaling activity in the indicated cells. *P < 0.05. c and d Analysis of miR-133a-3p expression with EGFR, FGFR1, IGF1R and MET, and p-AKT expression in 4 bone metastatic PCa tissues (T1–4) and 4 non-bone metastatic PCa tissues (T5–8). U6 was used as the control for RNA loading. miR-133a-3p expression levels were normalized to that miR-133a-3p expression of sample one. Each bar represents the mean ± SD of three independent experiments. *P < 0.05. α-tubulin was used as loading control. e Relative expression levels of EGFR, FGFR1, IGF1R and MET, and p-AKT at S473 and p-AKT at T308 expression in PCa tissues.The expression levels of EGFR, FGFR1, IGF1R and MET, and p-AKT expression were quantified by densitometry using Image J, and normalized to the levels of α-tubulin respectively. The sample with the lowest expression of each protein was used as a standard