Fig. 2

Transfer of miR-9 derived from NPC cells to HUVECs via exosomes. a The nanoparticle concentration and size distribution of the exosomes derived from 5-8F/con and 5-8F/miR-9 cells. b Transfer of miR-9 derived from NPC cells to HUVECs via exosomes. HUVECs were cultured with PKH67-labeled exosomes derived from the parental cells (5-8F) transfected with Cy3-pre-miR-9. The Cy3-miR-9 signals were detected in the cytoplasm and nucleus of HUVECs (red), and green signals indicated PKH67-labeled exosomes. Cy3-miR-9 signals are colocalized with PKH67 in HUVECs (yellow). Nuclear counterstaining was performed using 4′,6-diamidino-2-phenylindole (DAPI) (blue). The scale bar indicated 10 μm. c The expression of miR-9 in the exosomes derived from 5-8F/con, 5-8F/miR-9, CNE1/con and CNE1/miR-9 cells. d The expression of miR-9 in HUVEC cells which were co-cultured with exosomes derived from 5-8F/con, 5-8F/miR-9, CNE1/con and CNE1/miR-9 cells respectively. e The expression of pri-miR-9 in HUVEC cells which were co-cultured with exosomes derived from 5-8F/con, 5-8F/miR-9, CNE1/con and CNE1/miR-9 cells respectively. **, P < 0.01