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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Exosomal miR-9 inhibits angiogenesis by targeting MDK and regulating PDK/AKT pathway in nasopharyngeal carcinoma

Fig. 5

Exosomal miR-9 derived from NPC cells inhibited angiogenesis by regulating the MDK-PDK/AKT signaling pathway. a Antibody array analysis of HUVECs co-cultured with exosomes derived from 5-8F/miR-9 or 5-8F/con cells. Blue and red squares indicated more than two-folds of changes in protein phosphorylation. b HUVECs were co-cultured with exosomes derived from 5-8F/miR-9 and 5-8F/con cells, or were transfected with siMDK or control, respectively. Then, the expression of P70S6K and PDK1 were analysed by western blot. c After inhibiting PDK1 in HUVECs with OSU-03012 (AR-12), cell migration was measured and quantified by Transwell migration assay. d After inhibiting PDK1 in HUVECs with OSU-03012 (AR-12), tubule formation of HUVECs was examined by in vitro tube formation assay and quantified for tubule length. **, P < 0.01

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