Fig. 1

Characterization of macrophages educated by CM. a. Representative morphological images of THP-1-derived macrophages educated by CM from HIOEC, Leuk1, SCC25, and Cal27 for 24 h. The cytoskeleton was labeled with Fluorescent 555-Phalloidin (Red), and Nuclei were labeled with DAPI (Blue). b. Representative Scatter plots showed CD86 and CD163 levels evaluated by flow cytometry in THP-1-derived macrophages educated by CM from HIOEC, Leuk1, SCC25, and Cal27 cells for 24 h. The histogram showed statistical analysis for percentages of CD86⁺ macrophages. c. Expression levels of TNFα, IL1β, and IL6 of THP-1 derived and PBMCs derived macrophages educated by CM from HIOEC, Leuk1, SCC25, and Cal27 for 24 h, with or without subsequent LPS and IFNγ stimuli for another 24 h, as determined by quantitative real-time PCR. Data were represented as the mean ± SD of three independent experiments, **p < 0.01