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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: M1-like tumor-associated macrophages activated by exosome-transferred THBS1 promote malignant migration in oral squamous cell carcinoma

Fig. 3

Exosomes from OSCC cell lines activated macrophages into the M1-like phenotype. a. Expression of representative M1 and M2 marker genes determined by qPCR in THP-1-derived and PBMC-derived macrophages after stimuli with exosome supernatants of SCC25 and Cal27 for 24 h. b. Heat-map of cytokine expression profiles assessed by Luminex assay. THP-1-derived macrophages were treated with CM from HIOEC, Leuk1, SCC25, and Cal27, and exosome supernatant from SCC25 and Cal27 cells for 24 h, respectively. All samples were run in triplicate. Colors illustrated fold changes (see color scale). Red: up-regulation; blue: down-regulation. c. Detection of IL1β, TNFα, and IL6 levels in culture supernatants of THP-1-derived and PBMC-derived macrophages under education of CM from HIOEC, Leuk1, SCC25, and Cal27 cells, and exosome supernatant from SCC25 and Cal27 for 24 h, respectively. Data were expressed as the mean ± SD from three experiments, **p < 0.01, a significant difference compared with control, CM-HIOEC and CM-Leuk1 treated groups

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