Fig. 7

Exosomal transfer of THBS1 from OSCC activated macrophages into M1-like phenotype. a-b. THP-1-derived macrophages were treated with OSCC- exosomes for 0.5 h, 1 h, 2 h, 3 h, 4 h, 5 h, 6 h, 12 h, 24 h, and 48 h, respectively. Transcription levels of THBS1 and PAI1 were determined by qPCR. Data are representative of three independent experiments. c. Transcription levels of THBS1 in HIOEC, Leuk1, SCC25, and Cal27 cells. d-e. Expression levels of THBS1 were quantified in cellular culture supernatants and exosome suspensions by Platinum ELISA assays, **p < 0.01. f. Representative immunohistochemical staining demonstrating THBS1 was expressed by cancer cells in primary OSCC sample (100×). The upper part from normal oral epithelial tissue served as control; the lower part from primary OSCC tissue. g. Heat-map from the UCSC Xena Browser based on the primary TCGA HNSCC cohort depicted the gene expression relationship between TNFα, IL1β, or IL6 and THBS1, **p < 0.01. h. Expression pattern of THBS1, IL1β, IL6, and TNFα in a validated primary OSCC cohort (n = 30).i-j. Transcription levels for TNFα, IL1β, and IL6 in THP-1-derived macrophages educated by CM and exosome supernatants from THBS1-sh SCC25 and Cal27 cells for 24 h, respectively. Scrambled as control, **p < 0.01