Skip to main content
Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Transgelin-2 is a novel target of KRAS-ERK signaling involved in the development of pancreatic cancer

Fig. 3

Transgelin-2 binds to ERK2 through its 29–31 aa. a HEK293T cells were co-transfected with either wild-type transgelin-2 (GFP-T2-wt) or 29–31 aa truncated mutant (GFP-T2-∆) and Flag-ERK2 for 48 h. Co-immunoprecipitation was performed by anti-Flag antibody. The immunoprecipitated proteins were analyzed by anti-GFP antibody to detect the presence of GFP-tagged transgelin-2. b KRAS wild-type cells (Hs 766 T and BxPc3) and mutant cells (SW1990 and Capan-1) were collected for co-immunoprecipitation assay. The cell lysates were immunoprecipitated by anti-transgelin-2 antibody. The immunoprecipitated proteins were analyzed by western blot using anti-ERK1/2 antibody

Back to article page