Fig. 1

Molecular analysis in KRAS-mut lung and pancreatic cell lines. a-d. Lung cancer cell line A549 and pancreatic cancer cell line MiaPaCa2 were treated with dabrafenib (a) and RAF265 (b) alone or in combination with trametinib (c-d) for 4 h at the indicated doses. The cells were lysed and analyzed by Western Blotting using antibodies specific for the indicated proteins. Western blot with Hsp-70 specific antibody is shown as protein loading and blotting control. e A549 cells were treated with dabrafenib (10 μM) or RAF265 (10 μM) for 4 h. Endogenous CRAF was immunoprecipitated and the immunocomplexes were blotted for BRAF or CRAF. BRAF and CRAF levels in total cell lysates are also shown