Fig. 4

Effects of single and combined MEK and RAF inhibition in LCSC. a and c. Cells obtained from lung cancer spheres (LCSC) dissociation were treated with trametinib (10 nM) and dabrafenib (10 μM) alone or in combination for 4 h. The cells were lysed and analyzed by Western Blotting using antibodies specific for the protein above indicated. Western blot with Tubulin specific antibody is shown as protein loading and blotting control. b and d. LCSC2 and LCSC3 cells were plated in 96-well flat-bottom plates; trametinib and dabrafenib were added at their final concentration (1–10 nM), as single agents or in a fixed dose-ratio combination (1:1000). The results represent the average ± SD of three independent experiments. CI were calculated by conservative isobologram analysis for experimental data and plotted against the fraction affected