Fig. 1

miR-589 is up-regulated in GC tissues and cell lines and associated with poor prognosis of patients with GC. a Data from TCGA showed miR-589 expression was markedly increased in GC tissues compared with normal tissues. b Relative expression levels of miR-589 in gastric cancer cell lines and a normal gastric epithelial cell line, GES-1, Student’s t-test, mean ± SD, ***P < 0.001. c The expression level of miR-589 was upregulated in GC tissues compared with corresponding non-cancer tissues. d The expression level of miR-589 was upregulated in metastatic GC (mGC) tissues compared with non-metastatic GC (nmGC) tissue. e Analysis of TCGA data showed Kaplan–Meier survival curves for GC patients with distinct expression level of miR-589. f Real-time PCR was performed to detect the mRNA expression of miR-589 in MGC803 and BGC823 cells, both transfected with miR-589 mimic or anti-miR-589, Student’s t-test, mean ± SD, *P < 0.05; ***P < 0.001