Fig. 2

miR-589 induces GC cell migration, metastasis and invasion in vitro and promotes GC cell metastasis in vivo. Metastasis (a) and invasion (b) in MGC803 and BGC823 cells increased after transfected with miR-589 and decreased after transfected with anti-miR-589 in a transwell assay, Student’s t-test, mean ± SD, ***P < 0.001. c Wound healing was promoted in miR-589-transduced cells and delayed in anti-miR-589-transduced cells after 48 h. Results were expressed as mean ± SD of migrated distance from three different points. Migrated distance = width (0 h) – width (48 h), ***P < 0.001. d In vivo metastasis assays using bioluminescence imaging to detect metastases of MGC803-luciferase cells transfected with Lv-miR-589 or Lv-control. e Representative hematoxylin and eosin staining of lung tissue from different experimental groups. Scale bar = 50 μm