Fig. 4

LIFR is the direct target of miR-589 and suppresses GC cell metastasis, invasion and migration. a miR-589 and its putative binding sequences in the 3’UTR of LIFR. A mutation was generated in the complementary site that bound to the seed region of miR-589. Luciferase reporter assay was used to determine miR-589 direct targeting the LIFR 3’UTR, Student’s t-test, mean ± SD, **P < 0.01; ***P < 0.001. b Real-time PCR and Western blot analysis were performed to detect the mRNA and protein expression of LIFR in MGC803 and BGC823 cells, both transfected with miR-589 mimic or anti-miR-589, Student’s t-test, mean ± SD, *P < 0.05; **P < 0.01; ***P < 0.001. c Kaplan-Meier survival plots showed that lower expression of LIFR resulted in a worse survival, HR = 0.7, P = 0.016. Transwell assay (d & e) and wound healing assay (f) of MGC803 and BGC823 cells were performed after transfected with LIFR or pENTER vector, Student’s t-test, mean ± SD, **P < 0.01; ***P < 0.001