Fig. 6

c-Jun stimulates miR-589 expression by binding to its promoter region. a & b Real-time PCR was performed to detect the mRNA expression of miR-589 in MGC803 and BGC823 cells treated with LIFR and LY294002, Student’s t-test, mean ± SD, **P < 0.01; ***P < 0.001. c Knocking down c-Jun expression by siRNAs inhibited miR-589 and c-Jun expression in MGC803 and BGC823 cells, Student’s t-test, mean ± SD, *P < 0.05; **P < 0.01; ***P < 0.001. d Schematic representation of the promoter regions of miR-589 with the putative c-Jun TFBSs. Relative luciferase activity of the indicated promoter vectors in 293 T, MGC803 and BGC823 cells transfected with c-Jun plasmids, Student’s t-test, mean ± SD, *P < 0.05; **P < 0.01; ***P < 0.001. e PCR gel showing amplification of c-Jun-binding sites after ChIP using antibody against c-Jun. The gel figures were accompanied by the locations of molecular weight markers. f A schematic for an atypical miR-589-LIFR–PI3K/AKT-c-Jun feedback loop