Fig. 4

circ-IARS increases RhoA activity via absorption and regulation of miR122. a The expression levels of miR-122-5p, miR-140-3p,miR-505-3p, miR-561-5p and miR-612 in circ-IARS-overexpressing and circ-IARS-depleted HUVEC were detected by qRT-PCR. b Prediction of miR-122-5P (miR-122) binding sites in circ-IARS and RhoA transcripts. c-d The psiCHECK2- circ-IARS plasmid and the psiCHECK2- circ-IARS mut plasmid were constructed to confirm whether circ-IARS acts as a ceRNA to the miR-122. e Expression levels of miR-122 in HUVECs up-regulated or down-regulated for circ-IARS or NC. f-g qRT-PCR was used to assess relative expression levels of RhoA (f) and ZO-1 (g) in HUVECs with circ-IARS overexpression or interference or circ-IARS and miR122 together. h Relative RhoA activity in HUVECs. i Protein levels of RhoA, ZO-1 and RhoA-GTP were measured by western blotting. j Expression levels of F-actin and focal adhesion were imaged by confocal microscopy. Scale bars = 100 μm. k the changes of F-actin expression in WB were consistent with in IFC. l The numbers of AsPC-1 and Hs 766 T cells that passed through the endothelial monolayer were measured in Transwell analysis. Scale bars = 50 μm