Fig. 5

circ-IRAS enters HUVECs via exosomes and increases endothelial monolayer permeability. a The expression level of circ-IARS in Hs 766 T was detected by qRT-PCR after transfected with circ-IARS over-expression plasmids or siRNA. Exosomes isolated from Hs 766 T cells transfected with a circ-IARS overexpression plasmid, siRNAs or control were co-cultured with HUVECs. b-e Expression levels of circ-IARS (b), miR-122 (c), RhoA (d) and ZO-1 (e) were measured by qRT-PCR. f Relative RhoA activity was measured in HUVECs. g Protein levels of RhoA, ZO-1, RhoA-GTP were analyzed by western blotting. h Confocal microscopy was used to image expression of F-actin and focal adhesion. Scale bars = 100 μm. i WB found that the change of F-actin is the same as that of IFC. j In the Transwell assay, the tumor cells that passed through the endothelial monolayer were imaged by fluorescence microscopy. Scale bars = 50 μm