Fig. 2

Effects of JS-K on cell morphology and MMP in SMMC7721 and HepG2 cells. The cells were treated with different concentrations of JS-K for 24 h. a The morphological observation were observed under a phase contrast microscopy (400 × magnification). b The morphological observation was determined by crystal violet staining assay (200 × magnification). c Morphological observation under fluorescence microscopy after Hoechst33342 staining. The arrows indicated the typical features of apoptotic cells (400 × magnification). d The change of MMP was detected by using JC-1 staining and analyzed by flow cytometric. The percentage in histogram of each profile represents the percentage of total cells with low fluorescence intensity. Data are mean ± SD. n = 3 for each concentration. *P < 0.05,**P < 0.01, vs. control group